Research progress of intraocular lens materials / 国际眼科杂志(Guoji Yanke Zazhi)

With the wide application of phacoemulsification and intraocular lens implantation, many cataract patients have effectively recovered vision. Postoperative intraocular lens opacification is an important indicator for evaluating biocompatibility, as it affects the visual quality of patients. The manifestation and risk factors of opacification vary among different materials used for intraocular lenses. However, better visual quality after surgery is not determined by a single factor. The material of the intraocular lens and the patient's response to the intraocular lens are all factors that affect the postoperative visual quality. With the continuous advancement of technology, an increasing number of new materials are being applied in the field of intraocular lenses. Fully understanding the characteristics of intraocular lens materials, selecting suitable intraocular lens for patients and reducing complications caused by materials will be beneficial to patients. The characteristics of different intraocular lens materials and the risk factors of opacification after intraocular lens implantation were discussed in this paper.

A research review of mechanism and genetic susceptibility of lens opacity induced by low-dose ionizing radiation / 中国辐射卫生

The lens of the eye has been recognized as one of the most radiosensitive tissues. It has been known that a radiation dose of 0.5 Gy or higher can induce radiation cataract. Studies have also demonstrated that long-term exposure to low-dose radiation can increase the risk of lens opacity. However, the specific biologic mechanism of lens opacity induced by low-dose ionizing radiation is still unclear. Many mechanism chains may induce opacity independently or jointly, such as genomic damage in lens epithelial cells, oxidative stress, defects in intercellular communication, and inflammatory reaction, and genetic and epigenetic factors may also play a role. This paper briefly reviews the mechanisms of low-dose ionizing radiation inducing lens opacity as well as the role of genetic susceptibility in its development and progression, in order to provide a scientific reference for the prevention and control of this disease.

Effect of age on reversible lens opacity and Na⁺-K⁺-ATPase expression induced by chloral hydrate in mice / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effect of age on the expression of Na⁺-K⁺-ATPase and acute reversible lens opacification induced by chloral hydrate in mice. <p>METHODS: Acute reversible lens opacification was induced by intraperitoneal injection of 4% chloral hydrate(400mg/kg)in 3-month-old(young group)and 24-month-old(old group)C57BL/6 mice. The lens opacification was graded at 10, 20, 30, 45, 60, 90, 120 and 150min after chloral hydrate injection. The histopathological changes of lens were observed by hematoxylin eosin staining, and the expression of Na⁺-K⁺-ATPase in lens was detected by immunohistochemistry. <p>RESULTS: The development of lens opacity is similar in young and old mice after chloral hydrate injection. The lens opacification in the young group appeared earlier, thicker and lasted longer than the old group. HE staining showed that many vesicles appeared in the cortex below lens epithelial cells(LECs), and the structure of superficial lens fiber cells were disordered after chloral hydrate injection. Immunohistochemical staining showed that the expression of Na⁺-K⁺-ATPase was positive in LECs and fibers. The expression of Na⁺-K⁺-ATPase in LECs were weak before chloral hydrate injection and up-regulated 45min after chloral hydrate injection in young and old groups. The up-regulation of Na⁺-K⁺-ATPase was stronger in the old group than in the young group. <p>CONCLUSION: Age may play a role in the acute reversible lens opacification induced by chloral hydrate in mice. The expression of Na⁺-K⁺-ATPase is involved in lens opacity induced by chloral hydrate.

Ocular lens opacity in residents of areas with high natural radiation background in Yangjiang City, China / 中国职业医学

OBJECTIVE: To investigate the effects of long-term and low dose ionizing radiation on ocular lens opacities of residents living in areas with high natural radiation background(HNRB) in Yangjiang City, China. METHODS: A total of 483 Han residents from Yangjiang City(HNRB area) and 517 from Enping City(control area) were selected as study subjects using a cluster random sampling method. Questionnaire survey and lens examination were carried out. The risk factors of lens opacity and its severity were analyzed by logistic regression analysis. RESULTS: The prevalence rates of lens opacity, cortical opacity and posterior subcapsular opacity in HNRB area were higher than those in control area(60.7% vs 51.6%, 53.4% vs 46.8%, 21.9% vs 9.3%, all P<0.05). There was no significant difference in karyotype turbidity between HNRB area and control area(52.4% vs 47.6%, P>0.05). After adjusting for confounding factors including age, gender, cardiovascular/metabolic diseases, smoking, alcohol drinking and tea drinking, the unconditional logistic regression analysis results showed that the risk of ocular opacity, cortical opacity and posterior subcapsular opacity in residents of HNRB area was higher than that in control area(all P<0.05). Multivariate disordered logistic regression analysis results showed that residents in the HNRB area had a higher risk of grade two karyotype turbidity than grade one karyotype turbidity(P<0.01). Ordered logistic regression analysis results showed that residents in HNRB area had an increased risk of developing severe cortical turbidity(P<0.01). CONCLUSION: Long-term and low dose ionizing radiation exposure may increase the risk of ocular lens opacity, especially cortical and posterior subcapsular cataract, and affect the severity of the disease.

Comparing the number of attempts required for complete nuclear chop using calibrated and conventional phacotip

Background: Phacoemulsification is a state of art technique with a steep learning curve the configuration of the phacotip affects the efficacy and execution of the nuclear chopping techniques. Inadequate penetration of phacotip may result in partial thickness nuclear cleavage with residual posterior plane and over enthusiastic penetration may result in posterior capsule rupture. This may be avoided if some estimate can be made preoperatively of the depth of penetration required to achieve full thickness crack.Methods: A total of 60 eyes of 60 patients with age related cataract with grade 4.0 to 6.9 (LOCS III) fulfilling the inclusion and exclusion criteria were included in the study after written informed consent. They were divided into two groups A and B with 30 patients each randomly using envelope method and were operated using conventional and calibrated phacotips respectively and the number of attempts required to achieve complete nuclear chop was noted.Results: In group A, vertical chop was safely and effectively done is 23 patients in just one attempt while it took 2 attempts in 6 patients and 3 attempts in 1 patient. In group B, it took just one attempt in 26 patients while 2 attempts were required in 4 patients. The two groups were comparable (p value 0.453) and was found to be statistically significant.Conclusions: With the use of a calibrated phacotip, the surgeon already knows to what depth he has to penetrate the tip into the center of nucleus depending upon the grade of cataract which results in safe and effective chop in minimum attempts where as in the conventional phacotip, it is more of a blind process. Hence the calibrated phacotip has taken the guess work out of question.

Lens changes in cardiovascular catheterization laboratories staff / Cambios en el cristalino en personal de laboratorio de cateterismo cardiovascular

Abstract Objective: To determine the association between occupational ionizing radiation exposure in the cardiovascular catheterization laboratories staff and development of lens changes. Methods: A cross-sectional study was made. Participants were recruited at the XIII Colombian Conference of Interventional Cardiology and SOLACI conferences in Bogotá - Colombia 2017. Informed consent was obtained from all study participants. Demographics and clinical data were collected. Associated lens changes were determined according to the ionizing radiation exposure status. Results: A total of 89 subjects (178 eyes) participated in the study. In general, the mean age was 40 years old, ranging from 19 to 78 years old. Distribution according gender was 55.3% male and 44.7% female. 31 subjects (62 eyes) were deemed to have not occupational exposure to radiation and 58 subjects (116 eyes) with a history of occupational radiation exposure. Exposed eyes had twice risk for present subcapsular posterior lens opacity compared to non-exposed eyes. The subjects with older age and physician role were associated to the presence of lens opacity, with a p value 0.001 in both cases. Likewise, exposed subjects with any lens opacity had a high mean occupational lifetime, with a p 0.001. Conclusions: This study confirms a statistically significant increase in radiation-associated subcapsular lens changes in the eyes of interventional cardiology staff compared to unexposed controls.

Resumen Objetivo: Determinar la asociación entre la exposición a radiación ionizante en el personal de las salas de cateterismo cardiovascular y el desarrollo de cambios en el cristalino. Métodos: Se hizo un estudio transversal. Los participantes fueron reclutados en el XIII Congreso Colombiano de Cardiología Intervencionista y las Conferencias SOLACI en Bogotá - Colombia 2017. Se obtuvo el consentimiento informado de todos los participantes. Se recolectaron datos demográficos y clínicos. Se determinó la asociación entre los cambios en el cristalino y la exposición a la radiación ionizante. Resultados: Un total de 89 sujetos (178 ojos) participaron en el estudio. En general, la edad promedio fue de 40 años, con edades comprendidas entre los 19 años y 78 años. La distribución según el género fue del 55,3% en hombres y el 44,7% en mujeres. Se consideró que 31 sujetos (62 ojos) no tenían exposición ocupacional a la radiación y 58 sujetos (116 ojos) presentaban exposición ocupacional a esta. Los ojos expuestos tenían dos veces el riesgo de presentar opacidad subcapsular posterior del cristalino en comparación con los ojos no expuestos. Los sujetos de mayor edad y con rol de médico se asociaron a la presencia de opacidad del cristalino, con un valor p de 0.001 en ambos casos. Del mismo modo, los sujetos expuestos con cualquier opacidad del cristalino tenían una vida laboral más larga, con una p 0.001. Conclusiones: Este estudio confirma un aumento estadísticamente significativo en la presencia de cambios en el cristalino en el personal de las salas de cateterismo cardiovascular en comparación con los controles no expuestos.

Analysis of lens of 1720 medical application radiology workers in Hangzhou / 中华劳动卫生职业病杂志

Objective@#To analyze the lens opacity of some hospitals in Hangzhou to provide evidence for further improvement of radiation protection.@*Methods@#Physical examination data of 1720 radiological workers who underwent occupational disease physical examination in our hospital on January1, 2016and December 31, 2017 were collected. Lens turbidity, gender, age, type of work, radiological working age and other influencing factors were statistically analyzed, and logistic regression analysis was used for multipactor analysis.@*Results@#A total of 112 cases of lens turbidity (turbidity rate 6.51%) , after lens turbidity, subcapsular majority (64 cases (57.14%) ) ; lens turbidity increased with age, and showed an increasing trend of radiation working age; the lens turbidity rate was different in different types of work, including nuclear medicine (23.33%) 、radiology (6.76%) 、interventional radiology (6.06%) 、dental radiology (4.26%) and radiotherapy (4.21%) . Type of work、age and length of service are risk factors for lens opacity; Age and type of work were independent risk factors for lens opacity.@*Conclusion@#The turbidity of lens of radiologcial workers is related to age and workering age. Radiological workers engaged in nuclear medicine should strictly strengthen radiation protection.

Effect of lens opacity on retinal oxygen saturation in patients with diabetic cataract / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effect of crystal turbidity on retinal oxygen saturation in patients with diabetic cataract. <p>METHODS:This was a cross-sectional study. Totally 68 patients with 68 eyes of diabetic cataract admitted to our hospital from June 2017 to December 2017 were selected as subjects. Retinal oximetry was used to measure the blood oxygen saturation of the retinal veins, veins, and their supraorbital, nasal, subnasal, and infraorbital branches. The objective scatter index(OSI)of the eye was measured by Visual Quality Analysis System II, and the degree of opacity of the lens was graded according to OSI. <p>RESULTS:The blood oxygen saturation of the retinal artery and its branches in this group were 101.39%±10.84%, 106.19%±11.40%, 103.22%±10.91%, 102.36%±20.31%, and 101.29%±13.88%, respectively. The oxygen saturation of the retinal vein and its branches were 62.51%±8.95%, 66.37%±10.74%, 64.81%±8.97%, 58.37%±13.85%, and 58.66%±19.94%, respectively. The difference between arteriovenous oxygen saturation was 40.72%±12.08%. In this group of patients, 68 patients with 68 eyes had an OSI value of 4.21±3.14. Among them, 15 eyes were turbid at the first stage, 14 eyes were turbid at level 2, 23 eyes were turbid at level 3, and 16 eyes were turbid at level 4. Pearson correlation analysis showed that the retinal veins, veins and their branches were negatively correlated with OSI(both <i>P</i><0.01). The difference in retinal arterial and venous oxygen saturation was positively correlated with OSI(<i>P</i><0.01). There were significant differences in the blood oxygen saturation between the retinal veins, veins and their branches in patients with different degrees of lens opacity(<i>P</i><0.05). The retinal arterial, venous and branch oxygen saturation of patients with opacity of lens 4 was significantly lower than that of patients with opacity of lens 1, 2, and 3, and the difference was statistically significant(<i>P</i><0.05). There were no significant differences in blood oxygen saturation between the patients with opacity of lens 1, 2, and 3(<i>P</i>>0.05). There was no significant difference in the difference of arteriovenous oxygen saturation between the patients with different degrees of lens opacity(<i>P</i>>0.05).<p>CONCLUSION: In patients with diabetic cataract, when the degree of lens opacity is 1 to 3, the degree of abnormality of retinal blood oxygen metabolism is not obvious. When the degree of lens opacity reaches 4, the blood oxygen saturation of the retinal veins, veins and their branches will decrease.

Follow-up observation of eye lens in ten victims accidentally exposed to 60Co radiation source / 中华放射医学与防护杂志

Objective To observe late effect of 60Co radiation accident on eye lens in the victims.Methods Medical observations of eye lens were performed on ten victims accidentally exposed to 60Co sources in four radiation accidents that have occurred from 1986 to 2000 in Henan Province.Pathological changes of the eye lenses were examined by using slit lamp microscopy after mydriasis with compound tropicamide.Results Of these ten victims, Liang in a radiation accident in Kaifeng (in 1986), Mei in Xinxiang (in 1999) and Xu in Xuchang (in 2000) all had typical radiation-induced cataract 2, 3 and 6 years after irradiation, respectively.Follow-up observation of the lens showed the punctate and/or granular opacities present in the eye posterior subcapsular of Yan in Kaifeng accident, Jie in Zhengzhou (1987), and Tian, Yong and Yi in Xinxiang accident, featuring the early changes of radiation-induced cataract, but the posterior subcapsular opacities were not observed in Wang and Min in Xinxiang accident.Conclusions Focus should be on the eye lens as the target organ of radiation exposure in long-term follow-up of victims accidentally exposed to radiation source.Severity of the lens opacity induced by ionizing radiation is closely associated with radiation doses.

Functional disability in community-dwelling elderly: the role of cataracts and contextual factors / Incapacidade funcional em idosos comunitários: o papel da catarata e dos fatores contextuais

Introduction Cataract arises as a risk factor for functional disability in elderly Objective: To determine differences in functional capacity and contextual factors associated with it in elderly affected and not affected by cataract and association between disability and cataracts.Methods A transversal-type observational study was carried out with 100 community elderly, divided into two groups: elderly affected (n = 50) and not affected by cataracts (n = 50). It was evaluated: activities of daily living (ADL) – Katz Index –, instrumental activities of daily living (IADL) – Lawton scale – and mobility – Short Physical Performance Battery. Functional disability was detected when the elderly were dependent or semi-dependent for one or more IADL or ADL and/or when they presented any difficulty in mobility. Data analysis included absolute and relative frequencies, Chi-squared or Fisher’s exact tests (p < 0.05) and Poisson regression.Results No statistically significant associations were observed between IADL, ADL or mobility disability and cataracts. A statistically significant difference was observed between the disabled elderly affected and not affected by cataract according to contextual factors for ADL regarding age (p = 0.037) and comorbidity (p = 0.037), for mobility regarding the practice of physical activity (p = 0.013), and for IADL (p = 0.001), ADL (p = 0.001) and mobility (p = 0.013) regarding the self-reporting of eyesight problems.Conclusions Besides cataracts, physical inactivity, comorbidity and functional aging itself are contributing factors to the process of disability in elderly, so, the physiotherapy is essential both to prevent as to reverse this process.

Introdução Catarata surge como fator de risco para a incapacidade funcional em idosos.Objetivos Determinar diferenças na capacidade funcional e nos fatores contextuais a ela associados em idosos com e sem catarata e associação entre incapacidade funcional e catarata.Métodos Estudo observacional transversal, realizado com uma amostra de 100 idosos comunitários, divididos em dois grupos: idosos com catarata (n = 50) e idosos sem catarata (n = 50). Foram avaliadas: atividades de vida diária (AVD) – Índice de Katz –, atividades instrumentais de vida diária (AIVD) – Escala de Lawton – e mobilidade – Short Physical Pherformance Battery. Incapacidade funcional foi detectada quando os idosos apresentassem dependência ou semi-dependência para uma ou mais AVD ou AIVD e/ou qualquer dificuldade na mobilidade. As análises dos dados incluíram frequência absoluta e relativa, testes Qui quadrado ou Exato de Fisther (p < 0.05) e análise regressiva de Poisson.Resultados Não houve associação estatisticamente significativa entre AIVD, AVD ou mobilidade e ter catarata. Diferença estatisticamente significativa entre os idosos incapacitados de G1 e G2 conforme os fatores contextuais ocorreram para faixa etária (p = 0.037), comorbidade (p = 0.037) e incapacidade para AVD; prática de atividade física (p = 0.013) e incapacidade para mobilidade e autorrelato de dificuldade de enxergar e incapacidade para AIVD (p = 0.001), AVD (p = 0.001) e mobilidade (p = 0.013).Conclusões Além de catarata, inatividade física, comorbidade e o próprio envelhecimento funcional são fatores contribuintes para o processo de incapacidade em idosos, assim, a fisioterapia é essencial tanto para prevenir como para reverter este processo.

Analysis of lens opacity among 730 radiation workers / 中华放射医学与防护杂志

Objective To explore whether low-dose occupational ionizing radiation exposure can affect the prevalence of lens opacity.Methods Annual occupational health checkup data of 1 007 radiation workers was taken from a provincial medical institution for the purpose of statistical analysis.Logistic regression analysis was used to estimate occupational exposure odds ratios (OR) of lens opacity,adjusted for age,gender and length of service.Eye lens opacity was grouped into cortical,nuclear and posterior subcapsular opacity according to the position of the opacity occurrence site.Opacity occurred in any one of the both eye lens was recorded as turbidity.Results Only 730 cases coupled with complete information could be used in the statistical analysis.The rate of lens opacity for all the radiation workers was 10.27％.The rates of lens opacity by exposure group were estimated to be 9.07％ for radiation diagnosis and therapy group,11.11％ for intervention group,18.18％ for nuclear medicine group,and 9.33％ for industrial application group,respectively.Compared with those in the radiation diagnosis and therapy group,the workers engaged in intervention medicine,or nuclear medicine,were more likely to suffer from the lens opacity in posterior subcapsular position.The OR and its 95％ confidence intervals were 3.00 (1.23-7.33),4.12 (1.68-10.11) for the workers in intervention medicine or nuclear medicine group.Conclusions Medical radiation workers,who were exposed to long-term low-dose of ionizing radiation,especially those who engaged in intervention or nuclear medicine,were at significantly higher risk to develop lens opacity.

Effect of low-dose radiation on ocular lens of radiation workers: Meta-analysis / 中华放射医学与防护杂志

Objective To comprehensively analyze the effect of low-dose radiation on the lens of the eye of radiation workers.Methods The papers dealing with the relationship between occupational radiation exposure and the lens of the eye were collected by retrieving documents of the domestic and foreign medical information databases with references to other papers.There were 28 papers finalized with 17 608 workers included in the Meta-analysis.Stata12.0 was used for Meta-analysis,Q-test and I2 statistic for heterogeneity test,and funnel regression method,Begg's rank method and Egger's regression method for publication bias.Results The pooling odds ratio (OR) opacity in radiation workers were 2.51 (2.01,3.13),4.03 (2.77,5.85),respectively.Conclusions Low-dose radiation may lead to negative impact on ocular lens under the current occupational protection conditions.The proportion of posterior subcapsular opacity in radiation-related cataract is higher than that in age-related cataract.It is important to strengthen radiation protection of ocular lens.

Acute reversible lens opacity affects retinal HRA+OCT imaging in mice / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To assess the affect of ketamine and xylazine (ketamine/xylazine) to the transient lens opacity in mice.METHODS: Kimba mice (n=10) and wild-type mice (wt, n=8) were sedated with intraperitoneal injection of ketamine (60mg/kg for mice) and xylazine (10mg/kg) at 4, 8, 12 and 16 weeks old respectively. Pupils were dilated with tropicamide 25g/L alone to allow imaging lens status and retina using Spectralis HRA+OCT. RESULTS: All Kimba mice and wt presented extreme proptosis, suppression of the eye-blink reflex, corneal surface drying and lens opacities which occurred as early as 21±6 minutes after giving anesthesia and the lens opacities lasted up to 150±24 minutes.CONCLUSION: Ketamine/xylazine can cause transient lens opacity that may be related with the drugs side-effect.

Clinical significance of using color Doppler ultrasound to diagnose senile cataract / 中国医师进修杂志

Objective To evaluate the diagnostic value of color Doppler ultrasound to diagnose senile cataract and complication.Method Color Doppler ultrasound were used to check and analyze 185 eyes senile cataract of 120 patients and were compared with slit-lamp.Results In initial phase of senile cataract,the accuracy of ultrasonography was lower than slit-lamp.The false negative ratio of ultrasound was 15.22%(7/46).In middle and last phase of senile cataract,the relevant ratio of ultrasonography Was 100.00%(139/139).Conclusions The diagnosis sensitivity of color Doppler ultrasound in senile cataract is similar with slit-lamp.Ultrasound is the first choice to diagnose senile cataract in middle and last phase,especially when it is not seen by funduscope.Color Doppler ultrasound is an important way to diagnose senile cataract and complication.

Vascular Endothelial Growth Factor (VEGF) and Advanced Glycation End Products (AGEs) Overexpression in the Retina and Serum and Lens Opacities of Streptozotocin-induced Diabetic Rats / 당뇨병

BACKGROUND: Vascular Endothelial Growth Factor (VEGF) and Advanced Glycation End products (AGEs) have been implicated in the development of diabetic retinopathy. In this study, we examined the expression of VEGF and AGEs in the retina and serum, apoptosis in the retina, and lens opacities in streptozotocin (STZ)-induced diabetic rats. METHODS: The localization of VEGF and AGEs in the retina of STZ-induced diabetic rats was determined by immunohistochemical analysis, and apoptotic cell death was assessed using the TUNEL assay. In the serum, STZ-induced diabetic rats were assayed for VEGF and AGEs by ELISA. Lenses were also isolated to detect the opacity. RESULTS: Expression of VEGF and accumulation of AGEs were significantly increased in the retinal ganglion cell layers (GCL) and nuclear cell layers (NCL) of STZ-induced diabetic rats compared to normal control rats. In addition to cellular expression, serum VEGF and AGEs levels were also increased significantly in STZ-diabetic rats compared to normal rats (both P < 0.001) and there was a significant correlation between the serum VEGF and AGEs levels (r = 0.504). The lens opaque density of STZ-induced diabetic rats were significantly higher than in normal rats (P < 0.001). CONCLUSIONS: AGEs could be involved in the development of diabetic retinopathy through the induction of VEGF. One could possibly correlate this lens opaque formation with elevation of AGE induced VEGF level. Thus, this study should be considered as a basic research for studying pathology of the retina and lens in diabetic experimental models.

A Case of Intralenticular Triamcinolone Complicated by Intravitreal Triamcinolone Acetonide Injection

PURPOSE: To report a case of intralenticular triamcinolone complicated by intravitreal triamcinolone acetonide (IVTA) injection. METHODS: A 56-year-old man who presented with lens opacity in the right eye following IVTA injection as a treatment for the central retinal vein occlusion with macular edema was referred to our clinic. RESULTS: The visual acuity in his right eye was hand motion, which was not corrected. A slit lamp examination showed that the intralenticular crystalloid powder in the right eye was likely triamcinolone acetonide. Intralenticular crystalloid powder was detected during the phacoemulsification and the posterior capsule of the lens had been already ruptured. CONCLUSIONS: Lens opacity can be complicated by IVTA through intralenticular injection of triamcinolone acetonide.

Electroretinograms in Cataract Patients: Changes in Electroretinographic Responses According to the Cataract Type and Severity

PURPOSE: To evaluate the changes of electroretinographic responses according to the lens opacity type and severity in cataract patients with normal retina. METHODS: Retrospective study was performed on 79 eyes of 79 patients who had undergone electroretinograms (ERG) before cataract surgery. The patients with high myopia, diabetes mellitus, hypertension or glaucoma were excluded. Cataracts were classified using Lens Opacities Classification System III (LOCS III). Whether the ERG results are different according to the location and severity of lens opacity was analyzed. RESULTS: Marked reduction in amplitude and delay in implicit time were observed in mature cataracts. Amplitudes of a- and b-wave were reduced significantly, especially a-wave in maximal combined response and b-wave in rod response (P<0.01). Among the 4 groups, excluding the mature opacity, amplitude was reduced in posterior subcapsular opacity. Implicit times were also delayed, but not significant. CONCLUSIONS: Lens opacity, especially posterior subcapsular opacity affects the ERG results significantly. These findings should be considered in evaluating the retinal abnormalities in patients with cataract.

The Reversible Y-Suture Lens Opacity Formation in Endotoxin Induced Uveitis Model

The present study was undertaken to find out the role of NO on cataractogenesis in the experimenally-induced uveitis(EIU) model. Nitrite and nitrate, stable oxidative products of nitric oxide(NO), were measured in the aqueous humor and the progression of inflammations and lens opacities were evaluated with slit lamp biomicroscope. Immunoperoxidase staining and immunofluorescent staining for inducible NO synthase(iNOS) and peroxynitrite were performed to confirm the site of production of NO and peroxynitrite. The grades of inflammation were peaked at 24 hours inflammation was gradually decreased after 48 hours and lens opacity after 72 hours. These changes returned to the baseline level by one week after LPS injection. Similarly, NO concentration in aqueous humor was peaked at 24 hours. And it was then decreased after 48 hours and returned to the baseline level by one week. These inflammatory signs and lens opacities were significantly decreased in NG-nitro-L-arginine methyl ester(L-NAME) administrated group. Inflammatory cells in anterior chamber, iris, and ciliary body expressed highly iNOS which was coincide with peroxynitrite immunolocalization. Therefore, these results suggest that cataract formation in EIU is related to the NO production in aqueous humor. Furthermore, lipid peroxidation by peroxynitrite is possibly related with cataractogenesis in EIU. But, we need a further evaluation to seek the relationship between cataractogenesis and increased nitric oxide concentration, combined with studies of other biochemical changes in anterior chamber and lens.

Anti-catarct Animal Test of Anglimingshi Capsules / 中成药

Objective: To investigate the effects of Anglimingshi Capsules on preventing and treating experimental catarct. Methods: The model of D galactose induced catarct in rats was established. The effects of Anglimingshi Capsules on the catarct advance, lens opacity, lens structure, MDA and the concentrations of disoluble protein and high weight protein of treated rats were examined.Results: Anglimingshi Capsules could delay the advance of catarct, lower lens opacity and lighten the injury of lens structure by D galactose. Anglimingshi Capsules also decreased the concentration of MDA, lightened the injury of lens by lipid peroxidation and decreaseed the concentrations of disoluble protein and high weight protein. Conclusion: The test provided the foundation for application of Anglimingshi capsule.

Morphological Classification of the Lens Opacity of Age-Related Cataract in One Area of Seoul

The epidemiological study to evaluate the prevalence rate and the morphological classification was performed in Do Bong-Gu, Seoul from May 9, 1989 to May 25, 1989. However, this report was a part of preliminary data to make a further population based epidemiological study to evaluate the prevalence rate and morphological classification of the age-related cataract in Korea. The results of this study are as follow: 1. The total examinees were 100;39 male and 61 female, and they were over 40 years of age who complained of recent visual acuity decrement. 2. 91 persons had lens opacity in different decement. 3. The prevalence rate of cataract was 89.7% in male and 93.4% in female and the sexual difference shows that the female had more higher frequency of lens opacity. 4. The classification of lens opacity according to the stage showed that the immature cataract was the most frequent type. The cortical cataract was the most frequent type and the mixed type was second in order by the morphological classification. 5. The prevalence rate of cataract in age had strong tendency to increase in older age and in 80s the rate was in 100%.